Heat Shock Protein 47, Recombinant, Human, aa18-418, His-Tag (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antig

Product Overview

• Article Name: Heat Shock Protein 47, Recombinant, Human, aa18-418, His-Tag (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antig
• Biozol Catalog Number: USB-H1834-20E
• Supplier Catalog Number: H1834-20E
• Alternative Catalog Number: USB-H1834-20E-100,USB-H1834-20E-500
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: WB

Product Description

Heat shock protein 47(HSP47), also known as Colligin or Serpin H1, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein acts as a molecular chaperone facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Recombinant human HSP47/Colligin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa18-418 with a His-Tag from human Heat Shock Protein 47, expressed in E. coli. Accession: NP_001226. Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 48.9
• NCBI: 001226
• UniProt: P50454
• Purity: 90% (SDS-PAGE under reducing conditions and visualized by coomassie blue stain)
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol