Hemagglutinin Tag, Control Peptide (YPYDVPDYA) (HA-tag)
Biozol Catalog Number:
USB-H1847-52L1
Supplier Catalog Number:
H1847-52L1
Alternative Catalog Number:
USB-H1847-52L1-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins. Source: A 9aa peptide sequence from hemagglutinin influenza virus (aa114-122, YPY DVP DYA). Molecular Weight: ~3kD Applications: Suitable for use in ELISA. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Use 50-100ng control antigen/well Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
3
Purity:
(HPLC)
Form:
Supplied as lyophilized powder from PBS, pH7.4. Reconstitute with 1ml sterile PBS.
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