Hepatitis E Virus, Mosaic, ORF2/ORF3, Recombinant (HEV)
Biozol Catalog Number:
USB-H1927-23
Supplier Catalog Number:
H1927-23
Alternative Catalog Number:
USB-H1927-23-100,USB-H1927-23-500
Manufacturer:
US Biological
Category:
Molekularbiologie
Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function. The protein is a mosiac of 12 immunodominant HEV ORF2-ORF3 fragments. Applications: Suitable for use in ELISA and Western Blot, excellent for detection of HEV with minimal specificity problems. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specificity: Immunoreactive with sera of HEV-infected individuals Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95% as determined by 10% PAGE (coomassie staining). GS-4B sepharose-affinity purificiation.
Form:
Supplied as a liquid in 25mM Tris-HCl, 1mM EDTA, 0.5M urea, 50% glycerol.
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