Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function. Recombinant protein corresponding to aa92-123 of HEV ORF3 fused with beta-galactosidase (114kD) at N-terminal, expressed in E. coli. Specificity: Immunoreactive with sera of HEV-infected individuals. Applications: Recombinant HEV Antigen may be used in ELISA and Western Blot, excellent for detection of HEV with minimal specificity problems. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 6 months after reciept at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95% (SDS-PAGE, Bradford)
Form:
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10mM beta-mercaptoethanol, 8M urea.
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