Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25-aa residues in humans and 25-aa in mouse. In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. Human Sequence: DTHFP ICIFC CGCCH RSKCG MCCKT (25-aa). ICIFC CGCCH RSKCG MCCKT (20-aa) Mouse Sequence: DTNFP ICIFC CKCCN NSQCG ICCKT (25 aa) 4 cys-Cys bridges Recommended Dilution: ELISA: 1ug/ml to coat plates. Antibody Blocking: 5-10ug peptide. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
2192
Purity:
95% by HPLC
Form:
Supplied as a liquid in PBS, pH 7.5.
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