| Control Peptide for H2034-80A (antiserum) and H2034-80 (affinity purified) Rubber (cis-1,4-polyisoprene), an isoprenoid polymer, is produced in about 2000 plant species. It is the raw material of choice for gloves, tiresand other industrial products. Allergy to natural rubber latex products has been recognized as a serious medical problem especially among health care workers and children with spina bifida. Interestingly, there appears to be significant clinical and immunochemical cross-reactivity between some latex proteins and allergens in certain fruits and vegetables, such as banana, kiwi, avocado, and potato, and patients with fruit and vegetable allergy may be at increased risk for reacting to latex proteins. Proteins leached from the gloves or extractable latex proteins, ranging in size from 5-200kD, have been shown to be involved in eliciting type I hypersensitivity. Hevea brasiliensis has been the only commercial sourceof natural rubber mainly because of its abundance in the tree,its quality, and the ease of harvesting. Latex is produced in laticifers, which are specialized structures that consist of anastamosed latex-producing cells. Harvested Hevea latex is a complete cytosol with high protein content. Several potential allergens have been identified in Hevea latex, including rubber elongation factor (REF or Hev b 1), hevamine-1,3-glucanase (Hev b 2), hevein preprotein (HEV1), a 24-kD rubber particle-associated protein (RPP) (Hev b 3), and a component of the microhelix protein complex (Hev b 4), a 16kD acidic protein Hev b 5, Hev b 6, and Hev b 7. Hevein is a chitin-binding protein of 43 amino acids found in the lutoid body-enriched fraction of rubber tree latex. A hevein cDNA clone (HEV1) encodes a putative signal sequence of 17 amino acids followed by a polypeptide of 187 amino acids. Interestingly, this polypeptide has two distinct domains: an amino-terminal domain of 43aa, corresponding to mature hevein, and a carboxyl-terminal domain of 144aa. Hevein is unusually rich in cysteine and glycine. It has been shown to inhibit the growth of several chitin-containing fungi. Many chitin-binding proteins contain 1-3 hevein like domains (HLD) arrange in tandem. Two Wound-induced genes (WIN1 and WIN2) from potato encode proteins with HLD located at the N-terminus. Source: The 43aa peptide corresponding to hevein (1) 100% conserved in Hevein, and prohevein. It is 88% homologous in pseudo-Hevein, 75% in WIN2, 70% in class I Chitinase, and WIN. Applications: Suitable for use in ELISA as a blocking peptide for H2034-80. Not suitable in Western Blot. Other applications not tested. Recommended Dilution: ELISA: 50-100ng/well. Optimal dilutions to be determined by the researcher. Specificity: H2034-80B is 100% conserved in Hevein, and prohevein. It is 88% homologous in pseudo-Hevein, 75% in WIN2, 70% in class I Chitinase, and WIN. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
