His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems. Applications: Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detected His tagged Akt1/PKBalpha in RIPA lysates of transfected HeLa cells. Immunocytochemistry: 2ug/ml showed positive immunostaining for His tagged Akt1/PKBalpha in transfected HeLa cells. Optimal dilutions to be determined by the researcher. Positive Control: RIPA lysates of transfected HeLa cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[HIS.H8]
Isotype:
IgG2b
Purity:
Purified.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
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