Synthetic (linear) peptide corresponding to aa1-21 of human histone H3 followed by a GG linker and biotinylated lysine (ART[dimethylK]QTARKSTGGKAPRKQLA-GGK-biotin). Applications: Suitable for use in Enzyme assay. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Solubilization Instructions: The solubility of peptides depends on the primary structure of the molecule. While most dissolve freely in aqueous media, there are many examples of poor solubility, especially with peptides that contain an excess of hydrophobic residues. Unless you know that the peptide is freely soluble in your intended solvent, we recommend the following procedure: Add a small quantity of sterile, distilled water. If the peptide does not dissolve, sonication may help. Alternatively, or subsequently, add a small volume of 10% aqueous acetic acid (for basic peptides) or aqueous ammonia (for acidic peptides). Once the peptide is dissolved, buffer or salt solution may be added to obtain the desired concentration. Sterile filter the final stock solution. Peptides are more stable under acidic conditions and we recommend a choice of pH between 3.0 and 6.0. If still unable to dissolve: 1. Hydrophobic peptides: Try 1mg in 1ml deionized water and vortex, then add DMF drop by drop and vortex until dissolved. 2. Basic peptides: Try 1% acetic acid solution. If peptide still doesnt dissolve, add DMF drop by drop and vortex to help dissolve. 3. Acidic peptides: Try 1% ammonium solution. If peptide still doesnt dissolve, add DMF drop by drop and vorex until dissolved.
Purity:
> 90%
Form:
Supplied as a lyophilized powder. Labeled with Biotin.
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