Peroxidase conjugated rgp120 HIV-1 IIIB (Baculovirus). Molar Ratio: 2-4 peroxidase molecules per molecule of gp120. Specificity: Peroxidase conjugated rgp120 IIIB binds to murine monoclonal antibodies of defined epitope specificity and human monoclonal/polyclonal antibodies in ELISA and Western ELISA. Biological Activity: Peroxidase conjugated rgp120 IIIB binds to immobilized human T-cell receptor CD4 in ELISA and Western ELISA. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:1000 range depending on the concentration of CD4 in the test system. Binding to murine and human monoclonal/polyclonal antibodies may be performed at higher dilutions. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography to 95% purity as determined by SDS-PAGE, reduced.
Form:
Supplied as a liquid in 50mM Tris, pH 8.0, 1% BSA, 0.01% Thimerosal. Labeled with HRP.
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