Fluorochrome/Protein Ratio: 2-4 Biological Activity: This FITC-conjugated gp120 binds to CD4 bearing cells in FACS. Applications: Suitable for use in Direct Immunoflouorescent staining, FACS, Immunohistochemistry and Dot Blot. Other applications not tested. Recommended Dilutions: Direct Immunofluorescence: 1:50 Dot Blot: 1:100 using purified anti-gp120 monoclonal or polyclonal antibodies. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable at -70C for 6 months after receipt. Dilute required amount only prior to immediate use. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by gel exclusion chromatography and UF concentrated.
Form:
Supplied as a liquid in 0.1M NaPO4, 50mM sodium chloride, pH 7.0. No stabilizers or preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
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