Recombinant HIV-1 IIIB gag p24 is a cytosolic protein produced in the Baculovirus Expression System. Quantitative determination of the protein is based on a sandwich capture ELISA using a qualified p24 preparation as a reference. The capture ELISA is sensitive to 1pg of p24, 1ng/ml of p24 shows an O.D. of 1.0 at 405nm under standard ELISA conditions. Applications: Suitable for use in ELISA, Western Blot and Cell Activation Assays. Other applications not tested. Recommended Dilutions: ELISA: 10-100ng Western Blot: 10-100ng Optimal dilution to be determined by researcher. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and to rabbit and human serum polyclonal antibodies in ELISA and Western Blot. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
98% Purified by cation/anion affinity and gel exclusion chromatography to 98% purity as determined by SDS-PAGE, reduced.
Form:
Supplied as a liquid in 20mM sodium acetate, 0.15M sodium chloride, pH 6.5. No stabilizers or preservatives added.
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