Recombinant p24 HIV-1 covalently coupled to FITC of 0.7nm diameter. Binds to Murine mAbs of defined epitope specificity and Human pAbs as determined by dot blot assays. Concentration: ~0.5mg/ml Coupling Ratio: ~2-4 FITC molecules per mole of p24. Applications: Suitable for use in Western Blot and Immunocytochemistry and T-Cell research. Other applications not tested. Recommended Dilutions: Western Blot: 1:50 Immunocytochemistry: 1:500 Optimal dilutions should be determined by the researcher Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Purity:
Purified by cation/anion affinity and gel exclusion chromatography to 98% purity as determined by SDS-PAGE, reduced.
Form:
Supplied as a liquid in 10mM Tris, pH 8.0. No stabilizers or preservatives added. Labeled with FITC.
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