Coupling Ratio: ~10-20 moles of biotin per mole gp160. Specificity: Biotin gp160 binds strepavidin-AP/HRP in ELISA and Western ELISA. Biological Activity: Biotin gp160 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent. Applications: Suitable for use in T-cell Research, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Capture ELISA: 0.1-10ng, using 100ng of CD4 in solid phase. Optimal dilutions to be determined by the researcher. Storage and Stability: Store at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography to 95% purity as determined by SDS-PAGE, reduced.
Form:
Supplied as a liquid in 0.1M Tris, 0.5% BSA, 0.05% tween-20, 0.01% Thimersol. Labeled with Biotin.
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