Full length recombinant protein corresponding to HIV-1 IIIB gp120 glycoprotein produced in the CHO Expression System. Biological Activity: This protein binds to human T-cell receptor CD4 as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. Applications: Suitable for use in ELISA and Western ELISA. Also suitable for use in CD4 binding and T-cell activation studies. Other applications have not been tested. Recommended Dilutions: ELISA: 10-100ng Western Blot: 10-100ng Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
120
Purity:
Purified by immunoaffinity chromatography to 95% purity as determined by SDS-PAGE, reduced.
Form:
Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
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