Protein contains the C-terminus of gp120 and most of gp41. Strain: BRU Isolate Applications: Antigen in ELISA and Western Blots. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
~95% (HPLC-C4, 10% PAGE). Purified by S-Sepharose, ceramic hydroxyapatite and S-300.
Form:
Supplied as a liquid in 20mM Tris HCl, pH 8.0, 8M urea. No preservative added.
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