tat HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size). Coupling Ratio: OD520nm = 10, protein content approximately 25ug. Specificity: Gold conjugated tat binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase Anti-tat in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
Purity:
Purified by ion affinity and reverse phase HPLC to 99% as determined by SDS-PAGE and HPLC.
Form:
Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Labeled with Colloidal Gold.
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