Full length (100 amino acids) Recombinant HIV-1 MN tat produced in the E. coli Expression System. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies (HIV-1 converted serum) in ELISA and Western Blot. Biological Activity: The biological specificity of this protein was determined by LTR-Beta galactosidase induction in MAGI and MAGI-CCR5 cells. Tat in 1-10ug/ml range induced Beta- galactosidase activity 30-fold over un-treated cells. Endotoxin: 0.01EU/mg (BioWhittaker Kinetic QCL Kit) Applications: Suitable for use in ELISA, Western Blot and Transcriptional activation. Other applications not tested. Recommended Dilutions: ELISA: 10-100ng Western Blot: 10-100ng Transcriptional activation: 1-10ug/ml. Dilute tat stock solution in saline-phosphate buffer (150mM sodium chloride, 50mM sodium phosphate, pH 6.5) immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR-dependent transcriptional activation activity. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
95% (SDS-PAGE and HPLC)
Form:
Supplied as a liquid in 0.2M KCl, 5mM glutathione. No stabilizers or preservatives added.
* VAT and and shipping costs not included. Errors and price changes excepted
