HEXIM1, Recombinant, Human (Hexamethylene Bis-acetamide Inducible 1, HEXIM1)
Biozol Catalog Number:
USB-H6185-15B
Supplier Catalog Number:
H6185-15B
Alternative Catalog Number:
USB-H6185-15B-100
Manufacturer:
US Biological
Category:
Molekularbiologie
HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation. Recombinant protein corresponding to human HEXIM1with a His-Tag, expressed in E. coli and purified by using conventional chromatography techniques. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
44.8
Purity:
~90% by SDS-PAGE.
Form:
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 10% glycerol.
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