HMG1, Recombinant, Human (HMGB1, High Mobility Group, HMG Transcription Factor Box1, Amphoterin, Sulfoglucuronyl Carbohydrate Binding Protein, SBP-1, HMG3)
Biozol Catalog Number:
USB-H6202-01A
Supplier Catalog Number:
H6202-01A
Alternative Catalog Number:
USB-H6202-01A-100,USB-H6202-01A-500
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques. High mobility group box1 protein (HMGB1) aa1-215, human, His tagged, Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.