Fusion protein corresponding to amino acids 496-789 of human HIF-1 beta
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1, a nuclear protein activating gene transcription in response to reduced cellular 02 concentration, activates the transcription of VEGF, EPO, iNOS, heme oxygenase 1 and other intracellular responses critical to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. HIF-1a most closely resembles the Drosophila protein Sim. HIF-1b is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Applications: Suitable for use in Western Blot, ChIP, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:500, recognizes a band at approximately 92kD representing HIF-1Beta. ChIP: 1:10-1:500 Immunohistochemistry (Paraffin): 1:100 Optimal dilution determined by the researcher. Positive Control: MCF7 human nuclear extracts Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
