Recombinant human ARNT, aa517-691, P27540 (E. coli).
ARNT (Aryl hydrocarbon nuclear translocator, also HIF-1 beta) is an 87kD member of the bHLH-PAS subclass of the bHLH family of transcription factors. It is widely expressed, and serves as part of both the NF kappa B and aryl hydrocarbon receptor (AHR) signaling nodes. For the AHR, the AHR is normally inactive and bound to hsp90 in the cytosol. Following ligand binding, the AHR complex enters the nucleus, dissociates from hsp90, dimerizes with ARNT, and then binds response elements of select genes. Human ARNT is 789aa in length. It contains a DNA-binding region (aa 87-143), two PAS domains (aa161-235 and 362-458) and one PAC region (aa424-467). There are multiple potential splice forms. Two alternate start sites are found at Met10 and Met354, there is a deletion of aa329-755 and 77-91, and an Ala substitution for aa 328-789. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
