Hypoxia Inducible Factor 2a (Hypoxia Inducible Factor 2 alpha, HIF-2a, Endothelial PAS Domain Containing Protein 1, EPAS1, EPAS-1, HIF-2 alpha, HIF2a, HIF1 alpha-like Factor, HLF, Member of PAS Protein 2, MOP2, PASD2), Rabbit10

Product Overview

• Article Name: Hypoxia Inducible Factor 2a (Hypoxia Inducible Factor 2 alpha, HIF-2a, Endothelial PAS Domain Containing Protein 1, EPAS1, EPAS-1, HIF-2 alpha, HIF2a, HIF1 alpha-like Factor, HLF, Member of PAS Protein 2, MOP2, PASD2), Rabbit10
• Biozol Catalog Number: USB-H9810-01E
• Supplier Catalog Number: H9810-01E
• Alternative Catalog Number: USB-H9810-01E-100
• Manufacturer: US Biological
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, IP, WB
• Immunogen: Synthetic peptide corresponding to the C-terminus of mouse/human Hypoxia Inducible Factor 2a

Product Description

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:200-1:1000. Detects a band at ~118kD. Immunoprecipitation: 5ug/1mg lysate Immunohistochemistry (Paraffin): 1:100 Immunofluorescence: 1:100 Immunocytochemistry:1:100 Optimal dilutions to be determined by the researcher. Positive Controls: RCC4 Lysate, COS-7 Hypoxic Induced Control Lysate and PC-12 Nuclear [Hypoxic and Normoxic] Lysate Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Purified by immunoaffinity chromatography.
• Form: Supplied as a liquid in Tris-glycine, pH 7.5, 150mM sodium chloride, 0.05% sodium azide.