Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/mL detection limit for rhIBSP ~ 6.5ng/well. Western Blot: 0.1-0.2ug/mL detection limit for rhIBSP ~ 50ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
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