ICOS IgG2a Fc, Fusion Protein (Inducible Costimulator) (Biotin)
Biozol Catalog Number:
USB-I0649-80A
Supplier Catalog Number:
I0649-80A
Alternative Catalog Number:
USB-I0649-80A-25
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, FC
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Each secondary signal from CD28 or ICOS results in a discrete cytokine secretion profile displayed by the activated T cell. 1 Both activation processes are effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be a ligand for ICOS. Functional Application: ICOS-muIg binds to recombinant GL50-muIg in EIA. Human ICOS-muIg/Biotin Fusion Protein* Molecular Structure: A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight is 42.8kD (amino acid composition only). Transfectant Cell Line: CHO Applications: Suitable for use in Flow Cytometry, ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
40.4
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.
* VAT and and shipping costs not included. Errors and price changes excepted
