Recombinant protein corresponding to Ala26-Pro653 from human alpha-L-Iduronidase, expressed in NS0 cells.
Alpha-L-Iduronidase (IDUA) is a ubiquitously expressed lysosomal glycosyl hydrolase. It hydrolyzes the unsulfated terminal alpha-L-Iduronic acid residues in glycosaminoglycans including dermatan sulfate and heparin sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis. The amino acid sequence of rhIDUA is 79% and 61% identical to that of mouse and chicken, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using I0706-94 (recombinant human alpha -L-Iduronidase/IDUA). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
