Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000 to 1:30,000 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Biotin conjugated.
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