Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000. Western Blot: 1:1,000-1:5,000 Immunohistochemistry: 1:500-1:3,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with HRP.
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