Applications: Suitable for use in Immunofluorescence and FLISA. Other applications have not been tested. Recommended Dilution: IF Immunomicroscopy: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Label: Fluorescein isothiocyanate (FITC) Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of rabbit IgA Absorption Wavelength: 495nm Emission Wavelength: 528nm Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form:
FITC conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
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