Purified free human secretory component isolated from pooled milk
Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen Immunofluorescence: Indirect Optimal dilutions to be determined by the researcher. Fluorescein/IgG protein molar ratio (F/P): ~1.6 Excitation: 492nm Emission: 515nm Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light.
Purity:
Purified by DEAE chromatography
Form:
Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
* VAT and and shipping costs not included. Errors and price changes excepted
