IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma, found at a concentration of 0.4 to 2.2mg/ml. It plays a very important role in the first specific defense against natural infection. Secretory IgA differs from serum IgA in that it contains two additional peptides: the secretory component and the J chain. Applications: Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal working dilutions to be determined by researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
