IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma, found at a concentration of 0.4 to 2.2mg/ml. It plays a very important role in the first specific defense against natural infection. Secretory IgA differs from serum IgA in that it contains two additional peptides: the secretory component and the J chain. Applications: Suitable for use in Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen and Paraffin sections Optimal working dilutions to be determined by researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
