Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Horse IgG F(ab)2 molecule Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide.
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