Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome Protein Ratio: 5.0 moles FITC per mole of Guinea Pig IgG Applications: Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against aanti-Fluorescin, anti-guinea pig IgG and anti guinea pig serum.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
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