Applications: Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:4000 ELISA: 1:3000-1:25,000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in 0.05M Tris-chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.09% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP) (Calf Intestine MW:140kD
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