Applications: Suitable for use inFLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, containing 10mg/ml BSA (IgG and Protease free) and 0.01% sodium azide. Reconstitute in 1ml of ddH2O. Labeled with Fluorescein isothiocyanate (FITC).
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