Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry, Immunodiffusion and Conjugation. Other applications not tested. Recommended Dilution: ELISA: 1:20,000-1:100,000 Western Blot: 1:2000-1:10,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption.
Form:
Supplied as a sterile-filtered liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added.
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