Applications: Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form:
Fluorescein isothiocyanate (FITC) conjugated. Lyophilized in 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
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