Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA (standard capture): 1:50,000-1:250,000 Western Blot: 1:5000-1:25,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
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