Applications: Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide.
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