Applications: Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. Recommended Dilution: Assayed against 1ug of Rat IgG in a standard capture FLISA using peroxidase conjugated streptavidin. A working dilution of 1:12,000 to 1:50,000 is suggested. Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.6 moles Texas Red per mole of rabbit IgG Texas Red is a registered trademark of Molecular Probes Inc.
Purity:
99% Purified by Rat IgG-agarose affinity chromatography and solid phase adsorptio. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG and rat serum.
Form:
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with Texas Red sulfonyl chloride (MW: 625 daltons)
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