Applications: Suitable for use in FLISA (Fluorescent), Immunofluorescence, Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher. Absorption: 494nm Emission: 518nm FITC/Protein Ratio: 7.8 Moles FITC per Mole Antibody Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
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