Applications: Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by salt fractionation and ion exchange chromatography.
Form:
Supplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free). No preservative added. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
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