Applications: Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000 to 1:50,000 using Peroxidase Conjugated Streptavidin and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Reconstitution: Reconstitute with 500ul sterile dH2O.
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