Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule Applications: Suitable for use in immunoblotting, ELISA, immunohistochemistry, immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:15,000 to 1:60,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by Immunoaffinity chromatography
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide. Labeled with Biotin.
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