Applications: Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Label: Alkaline Phosphatase (Calf Intestine) (MW: 140kD) Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide and 50% glycerol. Labeled with Alkaline Phosphatase (AP).
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