Applications: Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Label: Fluorescein isothiocyanate (FITC) Molecular Weight: 390D Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 2.0 moles FITC per mole of Rabbit IgG Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Prepared by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label with Fluorescein isothiocyanate (FITC). Reconstitute with sterile dH2O or 40-50% glycerol for long term storage.
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