Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Label: Tetramethylrhodamine isothiocyanante (TRITC) (MW 444D) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.1 moles TRITC per mole of Rabbit IgG Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. TRITC conjugates are sensitive to light.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with1ml sterile dH2O. Labeled with tetramethylrhodamine isothiocyanante (TRITC).
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