Applications: Suitable for Immunoblotting (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:2000-1:4000 Use pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD) Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.
Form:
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphotase (AP).
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