Recombinant protein corresponding to Thr147-Pro325 from human IRF1 expressed in E. coli.
IRF1 (interferon regulatory factor 1) is a 45kD member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12p35. Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1-140) and an acidic C-terminus (aa 147-325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171-325, human IRF1 shares 83% aa identity with both canine and porcine IRF1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Reducing conditions. Validated with lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line and Jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
