Bovine Interleukin-1 beta (IL-1 beta) is expressed by a number of cell types including monocytes, macrophages and endothelial cells. This inflammatory cytokine has a wide range of effects, such as promoting T and B cell proliferation and inducing neutrophil chemotaxis. Recombinant protein corresponding to bovine IL-1 Beta, expressed in pichia pastoris Biological Activity: Recombinant Bovine IL-1 Beta has an ED50 in the range of 75-100pg/ml, determined by measuring the effect of recombinant bovine TNF IL-1 beta on the proliferation of murine D10.G4.1 cells. Applications: Suitable for use in Functional Assays. Other applications not tested. Endotoxin Level: <0.5EU/ug. Storage and Stability: Lyophilized and reconstituted products may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
